Regulatory

Part:BBa_K4611014:Design

Designed by: Saiyu Luo   Group: iGEM23_CJUH-JLU-China   (2023-10-01)


T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The three neighboring bases downstream of the T7 promoter have a large impact on promoter activity and are usually designed to be GGG to ensure T7 promoter activity.


Source

GenBank: Enterobacteria phage T7, complete genome.

References

Bacteriophage T7 transcription system: an enabling tool in synthetic biology. Wenya Wang , Yuwenbin Li , Yaqiong Wang , Chen Shi , Chenmeng Li , Qiang Li , Robert J. Linhardt.( https://doi.org/10.1016/j.biotechadv.2018.10.001)